Research (Professional)University of Pittsburgh

The laboratory studies musculoskeletal biology and tissue regeneration, focusing on skeletal and muscle stem/progenitor cells (SSCs and MuSCs). Projects aim to develop methods for tissue regeneration, differentiation, aging, and study responses to nutrients and supplemental peptides. Projects study skeletal stem cells with the eventual goal of high-level cell therapy design. Candidate will be experienced with flow cytometry, and basic cell biology/cell culture methods. We use time-lapse imaging, molecular assays, and cell-based analyses to explore population heterogeneity and lineage analysis, in the presence of oxidative stress and factors to mitigate that stress. The researcher will assist and/or lead ongoing projects involving molecular biology, spatial transcriptomics, and flow cytometry, contributing to experimental design, data collection, and analysis.

• Experience in flow cytometry, cell biology, molecular biology or equivalent research experience.
• Experience with primary cell culture or stem/progenitor cells, ideally muscle-related, or bone, ligament, tendon.
• Experience with murine models for cell transplantation/organoid generation.
• Demonstrated proficiency in molecular biology techniques (e.g., PCR, Western blotting, RNA/DNA isolation, cloning).
• Hands-on experience with flow cytometry and/or spatial transcriptomics, and an understanding of population heterogeneity.
• Strong data analysis, organizational, and communication skills.
• Ability to work independently and collaboratively in an interdisciplinary research setting.
• HIPAA and human subjects research certification required if working with identifiable human samples

The University of Pittsburgh is an equal opportunity employer / disability / veteran.