Laboratory Research Technician II - Di Luccia LabUniversity of Pittsburgh

The University of Pittsburgh, Department of Immunology, is seeking a full-time technician to support newly funded research programs. The Di Luccia Group investigates host-microbe- both commensal and pathogenic- interactions, focusing primarily on host mucosal immune responses. There are opportunities for independent projects within the mission of the laboratory, based on the applicant’s skills and goals, and after a period of guided supervision.

The applicant will support the function of the laboratory and assist the PI conducting experiments in cellular immunology and molecular microbiology. The applicant should have some experience with flow cytometry and basic immunological techniques. A significant task will include maintenance of the mouse colony, which includes breeding, PCR-based genotyping, and record keeping. The applicant should be very eager to learn new techniques and improve existing techniques.

Other required skills: Excellent organizational and planning skills, and attention to detail, are essential. Strong mathematical skills related to the biological sciences, including dilutions, ratios, concentrations, etc. Must keep detailed and accurate records. Excellent writing and verbal communication. Proven ability to work in a collaborative team, with some independent tasks. Experience with University of Pittsburgh systems and processes for purchasing and ordering is preferred.

Qualifications: A bachelor’s degree in Immunology, Biology or related field plus one (1) Year of Professional Experience is required in immunology/microbiology/molecular biology or other related fields. Knowledge of mathematical and chemical principles. Must be highly organized and efficient with their time. Must maintain professional demeanor and work well with others. Must be able to prioritize daily duties.

Performs a variety of laboratory experiments and procedures. Prepares specimens. Oversees supply management. Operates and properly maintains equipment.

1. Perform supervised laboratory work and experiments, including guided analysis and interpretation of primary cell culture experiments. These will involve cell isolation from organs, immunofluorescence staining, flow cytometry, cell sorting, cell culture, and other techniques. Write reports and summaries of findings with guidance from supervisor.

2. Perform cloning and basic microbiological techniques.

3. Oversees specimen inventories.

4. Maintains lab and chemical inventories. Assists with ordering as needed.

Work with supervisor to prepare data for presentation to the group and for publication.

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