2025-05-07
University of Pittsburgh
Other
/yr
employee
contract
Pittsburgh
Pennsylvania
15260
United States
Laboratory Research Technician
The Pulmonary, Allergy, Critical Care and Sleep Medicine Division of the DOM is hiring for a Laboratory Research Technician. The division is led by division chief Alison Morris, MD and Executive Administrator Barbara Suchonic. The division is made of up approximately 70 clinical staff and over 100 research staff who work in a variety of locations including: Bridgeside Point, Biomedical Science Tower (BST), UPMC Hospitals including Presbyterian, Shadyside, East, Jameson and Mercy. The division provides world class services across 10 inpatient services at five hospitals and over 70,000 outpatient office visits. We have a major research mission that focuses on basic, clinical, and translational research on the lung, microbiome, COVID-19, sleep medicine, and allergy and immunology.
The Division of Pulmonary within the Department of Medicine is seeking a laboratory Technician who will execute in vitro and in vivo lung injury and pneumonia murine and human models. Will perform detailed in vitro work using cell and tissue culture-based models including maintenance, transfection, and treatment of cell lines and primary cells; Will perform immunology, cellular and molecular biology techniques; Will be collaborative with trainees and colleagues within the laboratory group. Applicant must have at least one year of previous basic science laboratory experience.
Performs a variety of laboratory experiments and procedures. Prepares specimens. Oversees supply management. Operates and properly maintains equipment.
1) Wet bench responsibilities to include but not limited to ELISA assays; tissue harvesting and homogenate preparation; CFU counts from in vivo infection models; tissue slide preparation, sterile cell and tissue culture; DNA, RNA, and protein extraction and analysis; Western blotting, immunohistochemistry, flow cytometry, and immunofluorescence studies.
2) Management of mouse models of bacterial infection and execution of downstream experiments using these models.
3) Must have impeccable and timely record keeping skills which are required for experiment protocols and data generation for publications and grants.
4) Laboratory maintenance tasks such as order supplies, restocking common reagents, and maintaining the cell culture animal room spaces on a rotating basis with other lab members.
Physical effort requirements include ability to work at a laboratory bench for 8 hours a day for the full work week and standing for 1-2 hours for vivarium duties at least 3 times a week.
The University of Pittsburgh is committed to championing all aspects of diversity, equity, inclusion, and accessibility within our community. This commitment is a fundamental value of the University and is crucial in helping us advance our mission, which includes attracting and retaining diverse workforces. We will continue to create and maintain an environment that allows individuals to discover, belong, contribute, and grow, while honoring the experiences, perspectives, and unique identities of all.
The University of Pittsburgh is an Affirmative Action/Equal Opportunity Employer and values equality of opportunity, human dignity and diversity. EOE, including disability/vets.
PI270352154
