2025-10-03
University of Pittsburgh
Other
/yr
employee
contract
Pittsburgh
Pennsylvania
15260
United States
Noyes Lab Laboratory Research Technician II
Qualifications include: Bachelor’s degree with 1-2 years relevant work experience. Excellent verbal and written communication skills. Theoretical and practical knowledge of molecular cloning techniques is essential (DNA/RNA isolation, restriction digestion, PCR amplification, purification, etc.). Experience with human cell culture OR the handing/manipulation of bacteria is essential. Proficiency with basic computational tools used in biology (Excel, BLAST, primer design software) is essential. Experience with the preparation and quality control of Illumina sequencing libraries would be an asset. Experience with flow cytometry and FACS would be an asset.
Performs a variety of laboratory experiments and procedures. Prepares specimens. Oversees supply management. Operates and properly maintains equipment.
The Noyes lab works at the boundary of systems biology and synthetic biology to develop novel tools for genome editing, epigenetic editing, and cellular reprogramming. The Noyes Lab is seeking someone motivated for the position of Wet Laboratory Research Technician II. The successful candidate will be an integral part of a dynamic, interdisciplinary team of researchers, graduate students, and postdocs, investigating how common proteins function in order to predict the consequence of a mutation, and to develop synthetic version of these proteins for therapeutic applications. The Wet Laboratory Research Technician II will work closely with students and staff in the lab to assist in the prioritization of experiments. The Wet Laboratory Research Technician II will play an important role facilitating the day-to-day operations of the lab and will be required to perform moderately complex research activities.
Responsibilities include: Experimentation using human cell culture (routine handling, transfection, flow cytometry). Manipulating and screening large libraries of bacterial and yeast cells. DNA isolation from cells, PCR amplification, and gel purification of libraries. Preparing and processing of DNA libraries for sequencing using high throughput sequencing platforms (e.g., Illumina and PacBio). Maintaining laboratory stocks of media supplies, reagents, buffers, plates, etc. Performing routine maintenance of laboratory instruments. Assisting in analyzing and summarizing data for scientific communications (e.g., grant applications, peer-reviewed publications). Maintaining records of experimental data and communicating results to lab members. Reviewing pertinent scientific literature.
Physical Effort associated with wet lab work, i.e. standing while conducting experiments, sitting while working on computer, walking back and forth in the lab to sinks and microscopes, lifting packages less than 20lbs., etc.
PI278574104
